Journal: Materials Today Bio

Article Title: 3D bioprinted scaffolds for osteochondral regeneration: advancements and applications

doi: 10.1016/j.mtbio.2025.101834

Figure Lengend Snippet: Schematic diagram of 3D bioprinting for osteochondral tissue engineering. BMP: bone morphogenetic protein; TGF-β: transforming growth factor-β.

Article Snippet: For example, Li et al. employed 3D bioprinting to fabricate a biomimetic triphasic osteochondral scaffold [ ].

Techniques:

Journal: Materials Today Bio

Article Title: 3D bioprinted scaffolds for osteochondral regeneration: advancements and applications

doi: 10.1016/j.mtbio.2025.101834

Figure Lengend Snippet: Schematic representations of the strategies currently used in 3D bioprinting for osteochondral tissue regeneration. (A) Extrusion-based bioprinting. From left to right: pressure-based, piston-based, and screw based strategies. (B) Inkjet-based bioprinting using (left) thermal or (right) piezoelectric actuation. (C) Laser-based bioprinting. (D) Stereolithography-based bioprinting. Reproduced with permission . 2019, Wiley.

Article Snippet: For example, Li et al. employed 3D bioprinting to fabricate a biomimetic triphasic osteochondral scaffold [ ].

Techniques:

Journal: Materials Today Bio

Article Title: 3D bioprinted scaffolds for osteochondral regeneration: advancements and applications

doi: 10.1016/j.mtbio.2025.101834

Figure Lengend Snippet: 3D bioprinting of heterogeneous constructs providing tissue-specific microenvironment based on host-guest modulated dynamic hydrogel bioink for repairing osteochondral defects. (A) schematic illustration showing the fabrication process of network tethered host-guest modulated double-network hydrogel (MeHG DN). (B) Schematic illustration of 3D bioprinting biphasic ADSCs co-culture constructs with biomechanical and biochemical stimuli to create the tissue-specific microenvironment. (C) Cell viability, proliferation and spreading assay of ADSCs in 3D bioprinted constructs after cultured for 7 days. (D) Chondrogenic and osteogenic-related gene expression of ADSCs in each construct. (E) The gross and MRI images of the repaired osteochondral defects at 12 weeks post-surgery in different groups. (F) Histological images of H&E staining and SO staining, and immunohistological staining for type II collagen. Ac-β-CD: Acrylate β-cyclodextrin; GelMA: Gelatin methacryloyl; HG: Host-guest; HG DN: Host-guest and covalent double networks; MeHG DN: Methacrylated host-guest and covalent double networks; ADSCs: Adipose-derived mesenchymal stem cells; KGN: Kartogenin; MLT: Melatonin; MRI: Magnetic resonance imaging. Reproduced with permission . 2022, Wiley.

Article Snippet: For example, Li et al. employed 3D bioprinting to fabricate a biomimetic triphasic osteochondral scaffold [ ].

Techniques: Construct, Co-Culture Assay, Cell Culture, Gene Expression, Staining, Derivative Assay, Magnetic Resonance Imaging

Journal: Materials Today Bio

Article Title: 3D bioprinted scaffolds for osteochondral regeneration: advancements and applications

doi: 10.1016/j.mtbio.2025.101834

Figure Lengend Snippet: 3D bioprinting of fibre-reinforced cartilaginous templates for the regeneration of osteochondral defects. (A) 3D printed polymer frames were combined with cells encapsulated in alginate. (B) Biochemical analysis at day 28 for DNA and sGAG. (C) Young's modulus normalised to day 0 of each experimental group. (D) Schematic diagram of the fabrication of bioprinted monophasic and biphasic scaffolds. (E) Staining of histological sections for H&E, Aldehyde Fuchsin, Picro Sirius Red and collagen types II, I and X. PCL: Polycaprolactone; PLA: Polylactic acid; PLGA: Poly(lactic acid-co-glycolic acid); MSCs: Mesenchymal stem cells; sGAG: Sulfated glycosaminoglycans; BMSCs: Bone marrow-derived mesenchymal stem cells; RGD:Arginine-glycine-aspartic acid; FPSCs: Infrapatellar fat pad derived stem/stromal cells. Reproduced with permission . 2020, Elsevier. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: For example, Li et al. employed 3D bioprinting to fabricate a biomimetic triphasic osteochondral scaffold [ ].

Techniques: Polymer, Staining, Derivative Assay

Journal: Materials Today Bio

Article Title: 3D bioprinted scaffolds for osteochondral regeneration: advancements and applications

doi: 10.1016/j.mtbio.2025.101834

Figure Lengend Snippet: 3D bioprinted biomimetic multilayer scaffolds coordinated with sleep-related small extracellular vesicles to enhance osteochondral regeneration. (A) Schematic illustration of biomimetic multiple scaffolds prepared by 3D bioprinting. (B) Compressive modulus of GelMA, HAMA, and HG hydrogels. (C) Transwell experiment and merge staining of BMSCs and C28/I2 cells to evaluate the effect of MT-sEV on cell migration and proliferation, respectively. (D) Alcian blue staining of chondrocyte spheres. (E) Safranin O staining of chondrocyte spheres. (F) H&E staining of cartilage defect repair in a rabbit model at 12 weeks. (G) Toluidine blue staining of cartilage defect repair in a rabbit model at 12 weeks. GelMA: Gelatin methacryloyl; HAMA: Methacrylated hyaluronic acid; PCL: Polycaprolactone; HAPUW: Hydroxyapatite ultralong nanowire; HG: Methacrylated hyaluronic acid and gelatin methacryloyl; MT: Melatonin; sEV: Small extracellular vesicles; MT-sEV: Sleep-related small extracellular vesicles. Reproduced with permission . 2024, Wiley. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: For example, Li et al. employed 3D bioprinting to fabricate a biomimetic triphasic osteochondral scaffold [ ].

Techniques: Staining, Migration

Journal: Visual Computing for Industry, Biomedicine, and Art

Article Title: Exploration of nature-based biomimetic approach in landscape architectural design: parametric study of candelabra model design

doi: 10.1186/s42492-020-00060-y

Figure Lengend Snippet: Design of a 3D candelabra model using a biomimetic approach

Article Snippet: The construction of this urban design model was presented as a 3D candelabra model. Fig. 13 Conceptual design of candelabra This research included the implementation of a two-step biomimetic approach.

Techniques: